Off-address data in NEB-1 and you will HeLa telephone contours Inverse nested PCR try did having fun with genomic DNA regarding twenty six puromycin-unwilling NEB-step one clones that happen to be extracted from two separate transfections (A) Schematic drawing appearing care about-ligation from PstI absorbed genomic DNA including integrated pattP4X-PGKssPuro target vector. Ranking of relevant primers (Puro rev24, Puro rev303, pr21, attP fwd) employed for inverse nested PCR to .